Protein synthesis by single ribosomes

Protein synthesis by single ribosomes.

The ribosome is universally responsible for synthesizing proteins by translating the genetic code transcribed in mRNA into an amino acid sequence. Ribosomes use cellular accessory proteins, soluble transfer RNAs, and metabolic energy to accomplish the initiation, elongation, and termination of peptide synthesis. In translocating processively along the mRNA template during the elongation cycle, ...

Single-molecule imaging of full protein synthesis by immobilized ribosomes

How folding of proteins is coupled to their synthesis remains poorly understood. Here, we apply single-molecule fluorescence imaging to full protein synthesis in vitro. Ribosomes were specifically immobilized onto glass surfaces and synthesis of green fluorescent protein (GFP) was achieved using modified commercial Protein Synthesis using Recombinant Elements that lacked ribosomes but contained...

Ribosomes and protein synthesis

Let us start at the very beginning. Between 1897 and 1899, G. Gamier, in France, published elegant microscope studies describing a basophilic component ofthe cytoplasm of glandular cells (1) . Because of what he thought its role might be in the elaboration and transformation of secretory products, he gave a Greek name to these concepts-ergastoplasm (work plasm) . Gamier's research was extended ...

Synthesis of Nascent Protein by Ribosomes in Escherichia Coli.

Ricin inhibition of in vitro protein synthesis by plant ribosomes.

In vitro translation systems were prepared with supernatant factors from wheat germ and 80S ribosomes from wheat germ, barley embryos, watermelon cotyledons, pea cotyledons, and castor bean endosperm. Ricin A-chain, which strongly inhibits protein synthesis by mammalian ribosomes, inhibited all of the plant ribosomal systems by 50% when present at 25-45 mug/ml- approximately 23,000 times the co...